Pulk1 s757

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August undefined, 2024

WebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, … WebMay 19, 2013 · Whole cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. (c) Wild-type or FIP200 -/- MEF were incubated under nutrient rich, amino acid deprived or Torin-1 (+T, an mTOR inhibitor) conditions.

Phospho-ULK1 (Ser757) Antibody Cell Signaling …

WebNational Center for Biotechnology Information WebUlk1 is phosphorylated at S757, while mTOR kinase activity is partially suppressed under hyperosmotic stress. (A) Wild-type MEFs and T24 cells were cultured with amino acid … camera javafx

FoxO1-AMPK-ULK1 Regulates Ethanol-Induced Autophagy in …

WebMay 19, 2013 · Whole-cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. WebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, S14) and PIK3C3/VPS34 (S164) phosphorylation as well … WebSep 17, 2024 · Interestingly, ULK1 S757 phosphorylation was not only maintained, ... (Figs. 2 and 6), signs of an mTORC1-dependent autophagy deficiency such as pULK1 S757 … camera jeedom

An AMPKα2-specific phospho-switch controls lysosomal targeting …

Recombinant Anti-ULK1 (phospho S757) antibody [EPR22265-9 …

WebPulk1 S757, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebJan 18, 2024 · Whole cell extracts from MEFs, ESCs, and iPSCs were harvested for western blot using anti-p-ULK1(S555), anti-p-ULK1(S757), and anti-ULK1 antibodies. ULK1 … camera jazz bolognaWebMay 21, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity. camera jeevi

"WebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin (used as loading control) with specific antibodies; H:ULK1-to-β-actin;I: pULK1-s757-to β-actin;J: pULK1-s555-to β-actin;K: pULK1-s317-toβ-actin.All proteins were normalized to β-actin. " - Pulk1 s757

Pulk1 s757

WebDownload scientific diagram SopB-mediated activation of mTORC1 inhibits autophagy. Detection of pULK1 (S757) (A) or LC3-I/LC3-II (B) in B cells infected with indicated … WebNov 12, 2024 · PVT elevates ULK1 mediated autophagy in PDAC cells. Western blot analysis of pULK1(S757), ULK1, Atg101, FIP200, Beclin-1, Atg5, and LC3A/B in (A) PO2 (B) BxPC3 (C) L3.6pl, (D) AsPC1, and (F ...

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WebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of … WebBoster Bio Anti-ULK1 (S757) Antibody catalog # A00584S757. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody.

WebNational Center for Biotechnology Information WebWhole cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. (c) Wild-type …

WebAll lanes : Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution Lane 1 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate Lane 2 : … WebAug 28, 2024 · The densities of p-ATG13, p-BECN1, and p-ULK1 S757 are quantified and showed in the right panel. f HeLa cells were treated with different concentrations of PKC …

WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 …

WebJun 29, 2024 · Detection of pULK1 (S757) (A) or LC3-I/LC3-II (B) in B cells infected with indicated Salmonella strains for 3 h. ( C ) CFUs recovered from B cells infected with Salmonella strains for 24 h and treated for 1.5 h with autophagy-inducer peptide D11(20 μM) or scramble peptide L11 (20 μM). camera jazzWebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin … camera jazz bologna programmaWebFurthermore, we monitored increased levels of pp70S6K (T389), pULK1 (S757), and pAKT (S473), suggesting increased mTOR signaling ( Figure 5K, Figure S5U, S5V). ... View in full-text Context 19 camera jerikaWebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of … camera jeep 2.5WebAug 11, 2015 · pULK1 (S757) pS6K (T389) S6K Rictor pRictor (T1135) pS6 (S235/236) B-- - MK PF R AS160 Akt pathway mTORC2 pathway mTORC1/S6K pathway C pSIN1 (T86) pAkt (T308) pS6 (S235/236) pNDRG1 (T346) 14-3-3 pPRAS40 (T246) Insulin Inhibitor PRAS40 Protor mTORC1 mTOR Raptor mLST8 Growth Protein Synthesis S6K … camera jeepWebFeb 13, 2024 · Primary antibodies against Met, pMet (Y1234/1235), p62, LC3, mTOR, pmTOR (S2448), pULK1 (S757), Atg5, Beclin-1, NBR1, and secondary horseradish peroxidase-conjugated goat anti-rabbit and anti ... camera jelloWebpULK1-S555. Data are shown as mean phosphorylation (arbitrary units) ± s.e.m.; n = 3. Statistical significance vs. vehicle-treated WT was calculated by one-way ANOVA with Dunnett’s multiple-comparisons test. Representative immunoblots are shown. C) HEK293T cells expressing FLAG-tagged a1b1g1 or a2b1g1 were treated with 250 nM rapamycin or camera jeddah