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Fitc偶联annexin-v凋亡检测试剂盒

Web3. 加入5-15µg纯化的重组Annexin V。注意，不同的细胞系，以及凋亡的不同阶段，其Annexin V位点 饱和所需要的纯化的重组Annexin V含量不同。某些情况下，为达到最好效果，可以减少细胞数量， 0.5´105个细胞加5-15µg纯化的重组Annexin V。 4. 轻轻混匀，室温反应15分钟。 5.

Annexin V-FITC 凋亡检测试剂盒 Sigma-Aldrich

WebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs ... WebThe Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution. fnt to baltimore https://hellosailortmh.com

eBioscience™ Annexin V Apoptosis Detection Kits

WebAnnexin V是一种磷脂结合蛋白，与磷脂酰丝氨酸有高度亲和力，故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。. 因此Annexin V被作为检测细胞早期凋亡的灵敏指标之一。. 碘化丙啶（Propidium Iodide, PI）是一种核酸染料, 它不能透过完整的细胞膜 ... WebGently mix and incubate at RT for 15 min in the dark. *For Annexin V-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1X Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 554060) into 100 µl of 1X Binding Buffer and add to the cell pellet. Gently mix the cells and incubate at RT for ... Webannexin v- fitc/pi 凋亡检测试剂盒.由晶抗生物整理，包括annexin v- fitc/pi 凋亡检测试剂盒.使用说明书、应用范围、使用注意事项及操作步骤等资讯。 欢迎莅临上海晶抗生物工程有限公司官方网站！ fnt to chicago

一文讲清楚：Annexin V凋亡实验注意事项 - 知乎 - 知乎专栏

Category:ApoDETECT Annexin V-FITC Kit - Thermo Fisher …

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Fitc偶联annexin-v凋亡检测试剂盒

BD559763Anne_价格-品牌-详情介绍_丁香通 - biomart.cn

WebBioLegend Web英文名字 LinKine™ FITC Labeling Kit. 中文名称 LinKine™ FITC 偶联试剂盒. 产品货号 KTL0210. 规格 3*20ug. 背景资料： 异硫氰酸荧光素（FITC）是荧光素的衍生物，广泛应用于流式细胞术。 FITC是用异硫氰酸酯反应性基团（-N = C = S）官能化的原始荧光素分子，取代结构底部 ...

Fitc偶联annexin-v凋亡检测试剂盒

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WebAnnexin V-FITC Microscopy Staining Protocol. 1. Wash cells twice with 1X PBS. 2. Wash cells once with 1X Annexin V Binding Buffer. 3. Stain cells with Annexin V-FITC diluted 1:10 in 1X Annexin V Binding Buffer for 15 min at RT. We typically use 2 ml total volume per Labtek II chamber slide (i.e., 200 µl Annexin V-FITC and 1.8 ml 1X Binding Buffer. WebFormat. Kit. Kit Contents. Contains: • Annexin V Binding Buffer (10X), 1 bottle (30 mL) • Annexin V-FITC conjugate, 1 vial (0.25 mL) • Propidium Iodide Staining Solution, 1 vial (0.5 mL) Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits. Product Type.

Web推荐的实验流程. FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required ... WebDocuments. 产品描述. 本试剂盒用于检测细胞凋亡早期的发生，其中Annexin V为胞内蛋白膜联蛋白家族成员，以钙离子依赖的方式选择性与磷脂酰丝氨酸 (PS)结合。. PS正常分布在细胞膜内侧，即与细胞浆相邻的一侧。. 在细胞发生凋亡的早期，不同类型的细胞都会把PS ...

WebANNEXIN V- FITC/PI 凋亡检测试剂盒用于细胞凋亡检测，Annexin V 是一种分子量为35.8 KD 的Ca2+依赖性磷脂结合蛋白，能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸（Phosphatidylserine, PS）高亲和力特异结合。 Web实验原理. Annexin V-PE / 7-AAD荧光双染细胞凋亡检测试剂盒，可用于检测悬浮细胞和贴壁细胞的凋亡。. Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 (PS) 有高度亲和力。. 当细胞发生凋亡时，膜内侧的磷脂酰丝氨酸 (PS) 外翻到膜表面，而被荧光染料PE ...

WebStaining. 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.

WebFlow Cytometric Analysis of Annexin V-FITC staining. Jurkat cells were untreated (left panels), treated for 5 hours (middle panels) or 18 hours (right panels) with 4μMCamptothecin (Sigma,Cat.No C9911). Cells were incubated with Annexin V-FITC and 7-AAD and analyzed by flow cytometry. Untreated cells were primarily Annexin V-FITC … greenways marion arWeb一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI)，用于标记细胞膜完全破坏 … greenways manor guesthouseWebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. greenways maternity home chippenhamWebTransfer 100 µL of cell suspension in 5 ml test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404). 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. greenways meadow runcornWeb在细胞凋亡过程中，PS转位到质膜的外叶，当钙存在时，可通过与荧光染料标记的Annexin V结合，通过流式细胞术和细胞成像进行检测。 BD Biosciences提供几种常见形式的Annexin V，如用于紫色激光的FITC、PE、BD Horizon™ BV421和用于紫外激光的BD Horizon™ BUV395。随着这些 ... greenways mechanical and electricalWebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to … greenways medicalWebMay 27, 2024 · Annexin V-FITC细胞凋亡检测试剂盒(Annexin V-FITC Apoptosis Detection Kit)是用FITC标记的重组人Annexin V来检测细胞凋亡时出现在细胞膜表面的磷脂酰丝氨酸的一种细胞凋亡检测试剂盒。可以使用流式细胞仪、荧光显微镜或其它荧光检测设备进行检测。 fnt to chicago flights